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trpm8 primary antibodies  (Alomone Labs)


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    Alomone Labs trpm8 primary antibodies
    a Average current traces of <t>TRPM8</t> menthol response before and after exposure to the compounds in HEK293T cells expressing human TRPM8 from automated patch clamp experiments. Each dot is when the pulse program is applied. 100 μM menthol was applied for 75 s 4 times before perfusing 0.03% DMSO (control) or 10 µM compounds for 15 min. 100 μM menthol was then applied with corresponding compounds twice for 75 s. One n is the sum of 20 cells from an amplifier on the plate. b Average ratio of menthol response from each compound in TRPM8 HEK293T cells. The ratio calculated from data in ( a ), where the last two menthol responses before compound application were averaged and compared to the two menthol responses after compound application. P -values were determined by ANOVA followed by Dunnett’s tests. Bars are means ± SD. c Quantification of western blot analysis of phospho-EGFR (left) and ERK (right) from HeLa cells treated with compounds for 15 min then stimulated with EGF for 1 min. n = 3, bars are means ± SD. All comparisons are not significant.
    Trpm8 Primary Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trpm8+primary+antibodies/Anti-TRPM8+(extracellular)+Antibody+-+Carrier+Free/pmc12881431-428-8-16
    Average 95 stars, based on 3 article reviews
    trpm8 primary antibodies - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "Pharmacological tools to modulate ordered membrane domains and order-dependent protein function"

    Article Title: Pharmacological tools to modulate ordered membrane domains and order-dependent protein function

    Journal: Communications Chemistry

    doi: 10.1038/s42004-025-01874-8

    a Average current traces of TRPM8 menthol response before and after exposure to the compounds in HEK293T cells expressing human TRPM8 from automated patch clamp experiments. Each dot is when the pulse program is applied. 100 μM menthol was applied for 75 s 4 times before perfusing 0.03% DMSO (control) or 10 µM compounds for 15 min. 100 μM menthol was then applied with corresponding compounds twice for 75 s. One n is the sum of 20 cells from an amplifier on the plate. b Average ratio of menthol response from each compound in TRPM8 HEK293T cells. The ratio calculated from data in ( a ), where the last two menthol responses before compound application were averaged and compared to the two menthol responses after compound application. P -values were determined by ANOVA followed by Dunnett’s tests. Bars are means ± SD. c Quantification of western blot analysis of phospho-EGFR (left) and ERK (right) from HeLa cells treated with compounds for 15 min then stimulated with EGF for 1 min. n = 3, bars are means ± SD. All comparisons are not significant.
    Figure Legend Snippet: a Average current traces of TRPM8 menthol response before and after exposure to the compounds in HEK293T cells expressing human TRPM8 from automated patch clamp experiments. Each dot is when the pulse program is applied. 100 μM menthol was applied for 75 s 4 times before perfusing 0.03% DMSO (control) or 10 µM compounds for 15 min. 100 μM menthol was then applied with corresponding compounds twice for 75 s. One n is the sum of 20 cells from an amplifier on the plate. b Average ratio of menthol response from each compound in TRPM8 HEK293T cells. The ratio calculated from data in ( a ), where the last two menthol responses before compound application were averaged and compared to the two menthol responses after compound application. P -values were determined by ANOVA followed by Dunnett’s tests. Bars are means ± SD. c Quantification of western blot analysis of phospho-EGFR (left) and ERK (right) from HeLa cells treated with compounds for 15 min then stimulated with EGF for 1 min. n = 3, bars are means ± SD. All comparisons are not significant.

    Techniques Used: Expressing, Patch Clamp, Control, Western Blot

    Related Articles

    Labeling:

    Article Title: Pharmacological Tools to Modulate Ordered Membrane Domains and Order-Dependent Protein Function
    Article Snippet: .. Cells were then labeled with either of two TRPM8 primary antibodies targeted to an extracellular epitope (Alomone, Cat. No. ACC-049 Abcepta, Cat. No. AP8181D). .. The Alomone antibody was used at a dilution of 1:100 while the Abcepta antibody was used at a dilution of 1:50 for 1 hr in the flow cytometry buffer.

    Article Title: Pharmacological tools to modulate ordered membrane domains and order-dependent protein function.
    Article Snippet: .. Cells were then labeled with either of two TRPM8 primary antibodies targeted to an extracellular epitope (Alomone, Cat. No. ACC-049 Abcepta, Cat. No. AP8181D). .. The Alomone antibody was used at a dilution of 1:100 while the Abcepta antibodywas used at a dilution of 1:50 for 1 h in the flow cytometry buffer.

    Article Title: Small-Molecule Modulators of Lipid Raft Stability and Protein-Raft Partitioning
    Article Snippet: .. Cells were then labeled with either of two TRPM8 primary antibodies targeted to an extracellular epitope (Alomone, Cat. No. ACC-049 Abcepta, Cat. No. AP8181D). .. The Alomone antibody was used at a dilution of 1:100 while the Abcepta antibody was used at a dilution of 1:50 for 1 hr in the flow cytometry buffer.

    Article Title: Pharmacological tools to modulate ordered membrane domains and order-dependent protein function
    Article Snippet: .. Cells were then labeled with either of two TRPM8 primary antibodies targeted to an extracellular epitope (Alomone, Cat. No. ACC-049 Abcepta, Cat. No. AP8181D). .. The Alomone antibody was used at a dilution of 1:100 while the Abcepta antibody was used at a dilution of 1:50 for 1 h in the flow cytometry buffer.



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    a Average current traces of <t>TRPM8</t> menthol response before and after exposure to the compounds in HEK293T cells expressing human TRPM8 from automated patch clamp experiments. Each dot is when the pulse program is applied. 100 μM menthol was applied for 75 s 4 times before perfusing 0.03% DMSO (control) or 10 µM compounds for 15 min. 100 μM menthol was then applied with corresponding compounds twice for 75 s. One n is the sum of 20 cells from an amplifier on the plate. b Average ratio of menthol response from each compound in TRPM8 HEK293T cells. The ratio calculated from data in ( a ), where the last two menthol responses before compound application were averaged and compared to the two menthol responses after compound application. P -values were determined by ANOVA followed by Dunnett’s tests. Bars are means ± SD. c Quantification of western blot analysis of phospho-EGFR (left) and ERK (right) from HeLa cells treated with compounds for 15 min then stimulated with EGF for 1 min. n = 3, bars are means ± SD. All comparisons are not significant.
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    a Average current traces of <t>TRPM8</t> menthol response before and after exposure to the compounds in HEK293T cells expressing human TRPM8 from automated patch clamp experiments. Each dot is when the pulse program is applied. 100 μM menthol was applied for 75 s 4 times before perfusing 0.03% DMSO (control) or 10 µM compounds for 15 min. 100 μM menthol was then applied with corresponding compounds twice for 75 s. One n is the sum of 20 cells from an amplifier on the plate. b Average ratio of menthol response from each compound in TRPM8 HEK293T cells. The ratio calculated from data in ( a ), where the last two menthol responses before compound application were averaged and compared to the two menthol responses after compound application. P -values were determined by ANOVA followed by Dunnett’s tests. Bars are means ± SD. c Quantification of western blot analysis of phospho-EGFR (left) and ERK (right) from HeLa cells treated with compounds for 15 min then stimulated with EGF for 1 min. n = 3, bars are means ± SD. All comparisons are not significant.
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    Image Search Results


    a Average current traces of TRPM8 menthol response before and after exposure to the compounds in HEK293T cells expressing human TRPM8 from automated patch clamp experiments. Each dot is when the pulse program is applied. 100 μM menthol was applied for 75 s 4 times before perfusing 0.03% DMSO (control) or 10 µM compounds for 15 min. 100 μM menthol was then applied with corresponding compounds twice for 75 s. One n is the sum of 20 cells from an amplifier on the plate. b Average ratio of menthol response from each compound in TRPM8 HEK293T cells. The ratio calculated from data in ( a ), where the last two menthol responses before compound application were averaged and compared to the two menthol responses after compound application. P -values were determined by ANOVA followed by Dunnett’s tests. Bars are means ± SD. c Quantification of western blot analysis of phospho-EGFR (left) and ERK (right) from HeLa cells treated with compounds for 15 min then stimulated with EGF for 1 min. n = 3, bars are means ± SD. All comparisons are not significant.

    Journal: Communications Chemistry

    Article Title: Pharmacological tools to modulate ordered membrane domains and order-dependent protein function

    doi: 10.1038/s42004-025-01874-8

    Figure Lengend Snippet: a Average current traces of TRPM8 menthol response before and after exposure to the compounds in HEK293T cells expressing human TRPM8 from automated patch clamp experiments. Each dot is when the pulse program is applied. 100 μM menthol was applied for 75 s 4 times before perfusing 0.03% DMSO (control) or 10 µM compounds for 15 min. 100 μM menthol was then applied with corresponding compounds twice for 75 s. One n is the sum of 20 cells from an amplifier on the plate. b Average ratio of menthol response from each compound in TRPM8 HEK293T cells. The ratio calculated from data in ( a ), where the last two menthol responses before compound application were averaged and compared to the two menthol responses after compound application. P -values were determined by ANOVA followed by Dunnett’s tests. Bars are means ± SD. c Quantification of western blot analysis of phospho-EGFR (left) and ERK (right) from HeLa cells treated with compounds for 15 min then stimulated with EGF for 1 min. n = 3, bars are means ± SD. All comparisons are not significant.

    Article Snippet: Cells were then labeled with either of two TRPM8 primary antibodies targeted to an extracellular epitope (Alomone, Cat. No. ACC-049 Abcepta, Cat. No. AP8181D).

    Techniques: Expressing, Patch Clamp, Control, Western Blot

    FIGURE 4 TRPM8 level determination and TRPM8-silencing cell function experiments. (A–D) TRPM8 inhibition in PLC/PRF/5 and HUH-7 cells transfected with shTRPM8 lentivirus was verified by Western blot and qRT-PCR. (E–I) Effect of TRPM8 loss-of-function on PLC/RPF/5 and HUH-7 cell proliferation was determined by colony formation assay (E), CCK-8 (F-G) and EdU assay (H, I). (J, K) The effects of TRPM8 inhibition on the cell migration and invasion of PLC/PRF/5 and HUH-7 cells were detected using Transwell assays. Scale bars: 100 μm. *p < 0.05.

    Journal: Cancer medicine

    Article Title: TRPM8 overexpression suppresses hepatocellular carcinoma progression and improves survival by modulating the RTP3/STAT3 pathway.

    doi: 10.1002/cam4.70109

    Figure Lengend Snippet: FIGURE 4 TRPM8 level determination and TRPM8-silencing cell function experiments. (A–D) TRPM8 inhibition in PLC/PRF/5 and HUH-7 cells transfected with shTRPM8 lentivirus was verified by Western blot and qRT-PCR. (E–I) Effect of TRPM8 loss-of-function on PLC/RPF/5 and HUH-7 cell proliferation was determined by colony formation assay (E), CCK-8 (F-G) and EdU assay (H, I). (J, K) The effects of TRPM8 inhibition on the cell migration and invasion of PLC/PRF/5 and HUH-7 cells were detected using Transwell assays. Scale bars: 100 μm. *p < 0.05.

    Article Snippet: Primary antibodies against TRPM8 (Zen- bioscience, Chengdu, China), RTP3 (Epigentek, USA), STAT3 (Proteintech, Wuhan, China) and GAPDH (Proteintech, Wuhan, China) were employed.

    Techniques: Cell Function Assay, Inhibition, Transfection, Western Blot, Quantitative RT-PCR, Colony Assay, CCK-8 Assay, EdU Assay, Migration

    FIGURE 6 AD80 promoted the expression of TRPM8 and inhibited the proliferation, migration and invasion of HCC cells in vitro. (A, B) Calcium imaging was used to detected the changes of the fluorescence intensity of WS12 (A) and AD80 (B) groups on time in SNU-449 cells. (C, D) Effects of WS12 (C) and AD80 (D) on the expression of TRPM8 in SNU-449 cells were detected by Western blot. (E-G) EdU (E) and CCK-8 assays (F) were used to detect the effect of WS12 on the proliferation of SNU-449. (G) The effects of WS12 on the cell migration and invasion of SSNU-449 cells were detected using Transwell assays. (H–J) EdU (H) and CCK-8 assays (I) were used to detect the effect of WS12 on the proliferation of SSNU-449. (J) The effects of AD80 on the cell migration and invasion of SNU-449 cells were detected using Transwell assays. Scale bars: 100 μm. *p < 0.05.

    Journal: Cancer medicine

    Article Title: TRPM8 overexpression suppresses hepatocellular carcinoma progression and improves survival by modulating the RTP3/STAT3 pathway.

    doi: 10.1002/cam4.70109

    Figure Lengend Snippet: FIGURE 6 AD80 promoted the expression of TRPM8 and inhibited the proliferation, migration and invasion of HCC cells in vitro. (A, B) Calcium imaging was used to detected the changes of the fluorescence intensity of WS12 (A) and AD80 (B) groups on time in SNU-449 cells. (C, D) Effects of WS12 (C) and AD80 (D) on the expression of TRPM8 in SNU-449 cells were detected by Western blot. (E-G) EdU (E) and CCK-8 assays (F) were used to detect the effect of WS12 on the proliferation of SNU-449. (G) The effects of WS12 on the cell migration and invasion of SSNU-449 cells were detected using Transwell assays. (H–J) EdU (H) and CCK-8 assays (I) were used to detect the effect of WS12 on the proliferation of SSNU-449. (J) The effects of AD80 on the cell migration and invasion of SNU-449 cells were detected using Transwell assays. Scale bars: 100 μm. *p < 0.05.

    Article Snippet: Primary antibodies against TRPM8 (Zen- bioscience, Chengdu, China), RTP3 (Epigentek, USA), STAT3 (Proteintech, Wuhan, China) and GAPDH (Proteintech, Wuhan, China) were employed.

    Techniques: Expressing, Migration, In Vitro, Imaging, Fluorescence, Western Blot, CCK-8 Assay

    FIGURE 7 Schematic depicting the possible mechanism of the TRPM8- mediated RTP3/STAT3 pathway in HCC.

    Journal: Cancer medicine

    Article Title: TRPM8 overexpression suppresses hepatocellular carcinoma progression and improves survival by modulating the RTP3/STAT3 pathway.

    doi: 10.1002/cam4.70109

    Figure Lengend Snippet: FIGURE 7 Schematic depicting the possible mechanism of the TRPM8- mediated RTP3/STAT3 pathway in HCC.

    Article Snippet: Primary antibodies against TRPM8 (Zen- bioscience, Chengdu, China), RTP3 (Epigentek, USA), STAT3 (Proteintech, Wuhan, China) and GAPDH (Proteintech, Wuhan, China) were employed.

    Techniques: